A Highly Sensitive Methylation Assay for Prostate Cancer Diagnosis.

ABSTRACT:

Purpose

Prostate cancer is a prevalent malignancy among males, necessitating precise diagnosis for effective treatment and prognosis. However, there is a lack of accurate, reliable, and cost-effective methods for precise diagnosis of prostate cancer.

Materials and methods

The bisulfite-treated DNA was amplified by a blocker strand-assisted methylation-specific PCR method, and the signal was amplified by a guiding strand-assisted enzyme/probe detection system. On this basis, an Optimized DNA Methylation Detection Assay was developed. Fifty-five prostate cancer patients and 24 healthy patients were selected for blood/urine sample testing to evaluate the clinical value of the assay.

Results

The experimental results showed that the detection limit of the Tri-Component Liq

SUBMITTER: Zhong X 

PROVIDER: S-EPMC12798395 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature