CGRP/TSP1 Signaling Dampens Corneal Inflammation and Fibrosis by Targeting A2M During Corneal Stromal Wound Healing.

ABSTRACT:

Purpose

To investigate the specific role of thrombospondin-1 (TSP1), encoded by the Thbs1 gene, in corneal stromal wound healing and to elucidate its underlying mechanism.

Methods

A corneal stromal injury model was established by removing the epithelium and superficial stroma. We assessed Thbs1 expression using single-cell sequencing and compared the corneal healing responses between wild-type and Thbs1-deficient (Thbs1-/-) mice. Bulk RNA sequencing was conducted to identify key downstream targets of Thbs1. The regulation of TSP1 by calcitonin gene-related peptide (CGRP) was investigated through intervening CGRP signaling in human telomerase-immortalized keratocytes and murine corneas.

Results

Thbs1 was highly expressed in corneal stroma and upregulated following inju

SUBMITTER: Ge H 

PROVIDER: S-EPMC12798754 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature