Characterization of fecal deglucuronidation activity in healthy subjects and in patients treated with fecal microbiota transplantation.

ABSTRACT: Gut bacterial β-glucuronidase (GUS) enzymes contribute to the intestinal toxicity and/or enterohepatic recycling of glucuronidated compounds by cleaving glucuronide conjugates excreted into the intestinal lumen. The activities and substrate specificities of several GUS isoforms have been recently described. However, the extent of intraindividual and interindividual variability in gut microbial deglucuronidation activity has remained poorly characterized. In this study, we used pan-GUS reporter substrates as well as drug and steroid glucuronides to study the deglucuronidation activities of fecal lysates produced from individual fecal samples from healthy donors (n = 12), and sequential samples collected from fecal microbiota transplantation (FMT) donors (n = 3) and patients with recurrent C

SUBMITTER: Uoti A 

PROVIDER: S-EPMC12799528 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature