Cell penetrating peptide-functionalized small interfering RNA nanoparticles knock down HER expression in breast cancer cells.

ABSTRACT: Efficient delivery of small interfering RNA (siRNA) remains a major challenge in gene therapy, particularly due to poor cellular uptake, enzymatic degradation, and endosomal entrapment of the cargo. Cell penetrating peptides (CPPs) offer a promising strategy for intracellular delivery of nucleic acids; however, peptide-based gene delivery carriers possess limited nucleic acid condensation capability, demonstrating reduced transfection efficacies in mammalian cells. This study aimed to enhance siRNA delivery efficacies of CPP-based siRNA therapeutics by combining nucleic acid condensation efficacies of methylated protamine with the cell permeation capabilities of cyclic peptides to improve physiological stability, cellular uptake, and gene silencing efficacies in HER2⁺ breast can

SUBMITTER: Dissanayake R 

PROVIDER: S-EPMC12799534 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature