Multi-faceted roles of β-arrestins in G protein-coupled receptor endocytosis.

ABSTRACT: The internalization of G protein-coupled receptors (GPCRs) is a key process limiting cell surface receptor activity, and possibly promoting their intracellular signaling. Dysregulation of this process has been implicated in various diseases. This process is known to result from the recruitment of β-arrestins (βarrs) to the active receptors, although some receptors do not require this for unclear reasons. Here, we clarify the importance of βarrs in the internalization of 60 different GPCRs. We show that βarrs are essential for agonist-induced internalization for only a third of the receptors, half of the GPCRs being partially dependent on βarrs or even independent for endocytosis. Furthermore, we develop several Förster resonance energy transfer (FRET)-based molecular interaction assays to

SUBMITTER: Liu J 

PROVIDER: S-EPMC12799616 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature