Ferroptosis inhibits cementoblast mineralization via cGAS-STING/GPX4 axis.

ABSTRACT:

Background

To explore the potential role of cGAS-STING/GPX4 axis-mediated ferroptosis in cementoblast mineralization under compressive force and to determine its involvement in orthodontically induced inflammatory external root resorption (OIIERR).

Methodology

An immortalized murine cementoblast cell line (OCCM-30) was subjected to a 2 g/cm² compressive force for 24 h to establish an in vitro loading model. Western blot was used to detect proteins associated with mineralization (RUNX2, OPN, OCN) and components of the cGAS-STING/GPX4 axis. Ferroptosis was assessed by measuring ROS, Fe²⁺, and MDA levels. Mitochondrial damage was examined via mitochondrial membrane potential analysis and mtDNA linkage evaluation. To further investigate the role of cGAS-STI

SUBMITTER: Wei T 

PROVIDER: S-EPMC12799880 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature