Bone marrow mesenchymal stem cell-derived circHECTD1 targets claudin1 through the CTCF/METTL3 axis to alleviate ulcerative colitis : Short title: BMSC-derived circHECTD1 alleviates UC.

ABSTRACT:

Objective

Ulcerative colitis (UC) is an inflammatory bowel disease that lacks satisfactory treatment. This study aimed to investigate the role of bone marrow mesenchymal stem cell-derived exosomal circHECTD1 (Exo-circHECTD1) in UC and its mechanism of action.

Methods

An inflammatory model was created using LPS-stimulated MODE-K cells and an UC mouse model was established using dextran sodium sulfate (DSS). Cell proliferation was assessed using CCK-8. Apoptosis and Th17/Treg cell differentiation were analyzed by flow cytometry. Inflammatory factors were detected using ELISA. FITC fluorescence intensity was measured to evaluate permeability. m⁶A modification and molecular binding were detected using immunoprecipitation methods. Luciferase reporters were used to eval

SUBMITTER: Wang K 

PROVIDER: S-EPMC12799886 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature