Quantification of esterified oxylipins following HILIC-fractionation of lipid classes.

ABSTRACT: Several oxylipins are lipid mediators derived from the oxidation of polyunsaturated fatty acids (PUFAs). The majority of oxylipins in biological samples occurs esterified in neutral lipids (nLs) and phospholipids (PLs). They are commonly quantified indirectly following alkaline hydrolysis, providing excellent sensitivity. However, the information in which lipid classes the oxylipins occur is lost. The direct analysis of oxidized lipids is currently not sensitive enough to detect all esterified oxylipins. Here, a new hydrophilic interaction liquid chromatography (HILIC)-based lipid class fractionation using solid-phase extraction (SPE) cartridges was developed, separating lipids into nLs and 4 PL fractions using a single column. Esterified oxylipins in the fractions were quantified followin

SUBMITTER: Wende LM 

PROVIDER: S-EPMC12799955 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature