Nitric Oxide-mediated S-nitrosylation of the Energy Sensor KIN10 Regulates RNA Splicing and Gene Expression in Arabidopsis.

ABSTRACT: Nitric oxide (NO) is a crucial signaling molecule involved in various developmental processes and stress responses through post-translational protein modification and modulation of gene expression. Despite significant advances in understanding the mechanism of NO-mediated protein modifications, how NO regulates gene expression remains largely unclear. Here, we show that the energy sensor KIN10, a catalytic α-subunit of sucrose non-fermenting 1-related kinase 1, plays a vital role in NO-mediated regulation of gene expression in Arabidopsis. NO-mediated S-nitrosylation at Cys-177 of KIN10 inhibits its degradation, leading to protein stabilization. A non-nitrosylatable mutation of Cys-177 to serine results in NO insensitivity and functional deficiencies. Quantitative phosphoproteomic analysis

SUBMITTER: Yi Y 

PROVIDER: S-EPMC12799962 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature