Plasma-based digital PCR assay for early detection of gastric cancer using multiple methylation biomarkers.

ABSTRACT: Endoscopy is the gold standard for diagnosing gastric cancer (GC), but its invasiveness limits widespread participation and has not substantially reduced GC-related mortality. This study developed and validated a blood-based digital PCR assay for early GC detection using DNA methylation biomarkers. Genome-wide methylation profiles from over 10,000 samples were screened, and two candidates were validated in GC cell lines, tumors, matched non-cancerous tissues, and plasma. Plasma from 60 GC patients, including 38 with stage I disease, and 40 healthy controls was analyzed with a digital PCR assay targeting the selected biomarkers, using ACTB as a reference. GHR and GLRB methylation were identified as novel GC biomarkers, showing consistent hypermethylation in GC cell lines and tumor tissues.

SUBMITTER: Lee YY 

PROVIDER: S-EPMC12800017 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature