Ginkgetin reverses cisplatin resistance in cervical cancer by regulating the Nrf2/HO-1 signaling pathway to induce ferroptosis.

ABSTRACT: This study investigates whether Ginkgetin(GK) reverses cisplatin(DDP) resistance in cervical cancer cells by modulating the Nrf2/HO-1 signaling axis to induce ferroptosis, and preliminarily elucidates its underlying mechanisms. DDP-resistant cervical cancer cell line HeLa/DDP was used for in vitro experiments. Cell proliferation was assessed by CCK-8 assay; migration and invasion capabilities were evaluated via wound healing and Transwell assays; colony formation assays measured proliferative capacity. Molecular docking analyzed the binding affinity of GK to Nrf2, HO-1, and GPX4 proteins. Intracellular ROS and Fe²⁺ levels were detected using fluorescent probes. Ferroptosis-related indicators including GSH, MDA, SOD, and CAT were measured by biochemical detection. Western blot an

SUBMITTER: Wang F 

PROVIDER: S-EPMC12800045 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature