Dynamic and functional changes in vascular endothelial cell monolayers under combined hypoxic and inflammatory stimuli.

ABSTRACT: In sepsis, dysregulated inflammatory responses cause microthrombi formation, leading to organ hypoxia. While endothelial cell (EC) responses to inflammation are well-studied, the combined effects of hypoxia and inflammation remain unclear. We investigated EC dynamics and function under simultaneous hypoxia and lipopolysaccharide (LPS) exposure using microfluidic devices with controllability of oxygen concentration. Collective cell migration was measured by particle image velocimetry, while monolayer permeability was assessed by diffusion of fluorescently labeled dextran. Additionally, we examined adhesion and signaling protein expression. Migration speed decreased under approximately 1% O₂, but LPS exposure delayed this decrease, attenuating hypoxic responses. Both hypoxia and L

SUBMITTER: Sone K 

PROVIDER: S-EPMC12800060 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature