METTL3/RBM15 augments the stability of Kdm6b mRNA and promotes STAT1-mediated macrophage activation and atherosclerosis.

ABSTRACT: Atherosclerosis is the underlying cause of cardiovascular disease. Recent studies have shown that N⁶-methyladenosine (m⁶A) modification in macrophages is associated with atherosclerosis progression. However, there is a lack of systemic research on the role of m⁶A modification in macrophage differentiation and activation during atherosclerosis. Here we conducted multiomics analysis (MeRIP-seq and RNA-seq) of macrophages during their differentiation and activation to elucidate the regulatory network of the m⁶A spectrum at different stages. Western blot, quantitative PCR (qPCR), RNA-seq and RNA immunoprecipitation (RIP)-qPCR results demonstrated that m⁶A modification modulates KDM6B expression during macrophage activation. Through co-immu

SUBMITTER: Huangfu N 

PROVIDER: S-EPMC12800227 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature