Plasma cell-free extrachromosomal circular DNA is a molecular hallmark of preeclampsia.

ABSTRACT:

Background

Extrachromosomal circular DNA (eccDNA) is emerging as a novel biomarker for multiple diseases, but its role in reproductive diseases remains underexplored. Preeclampsia (PE) is associated with increased maternal and perinatal morbidity and mortality. Identifying new screening biomarkers may improve PE-affected pregnancies' outcomes.

Methods

To investigate eccDNAs in PE, we collected plasma biopsies from 24 PE-affected and 23 normotensive (Norm) pregnancies, including 14 paired samples collected before and after diagnosis. eccDNA was profiled through linear DNA removal, circular DNA enrichment, and sequencing. The reliability of eccDNA detection was validated using Integrative Genomics Viewer plots and Sanger sequencing.

Results

Our analysis identified signi

SUBMITTER: Han P 

PROVIDER: S-EPMC12800372 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature