Aptamer-based approaches for sensitive detection and epitope mapping of SARS-CoV-2 spike protein.

ABSTRACT: The SARS-CoV-2 spike (S) protein, crucial for viral entry, remains a key target for diagnostics and therapeutics amid evolving variants. Here, we describe the selection and characterization of novel DNA aptamers targeting the S1 subunit, including the Omicron strain, via systematic evolution of ligands by exponential enrichment (SELEX) and biolayer interferometry (BLI). Three aptamers-AptS1-tSP4, AptS1-tSP10, and AptS1-tSP11-exhibited nanomolar dissociation constants (14-59 nM), with AptS1-tSP10 demonstrating good selectivity over MERS-CoV and robust binding in human saliva and pseudovirus samples. Integration with proximity ligation assay and qPCR (PLA-qPCR) achieved a detection limit of 3 pM, surpassing many antibody-based methods. Mass spectrometry-based epitope mapping identified the r

SUBMITTER: Poolsup S 

PROVIDER: S-EPMC12800406 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature