Methodology of DNA extraction and sequencing from living cardiomyocytes collected by catheter in humans.

ABSTRACT:

Purpose

We present here the technical feasibility of percutaneously retrieving cardiomyocytes (CMs) through the lumen of irrigated ablation catheters, with the aim of obtaining DNA of sufficient quality/quantity for allowing DNA amplification, screening, and derived genetic analysis.

Methods

Irrigated conventional catheters for ablation were used for creating endocardial right ventricular voltage maps in 38 patients with suspected or proved arrhythmogenic right ventricular cardiomyopathy. Blood material was collected from scar areas by aspiration and filtered, CMs detected by light microscopy were aspirated, centrifugated, and freezed.DNA was extracted, amplified, and sequenced, and variants were compared with variants obtained from leukocyte DNA.

Results

At least 1 C

SUBMITTER: Ader F 

PROVIDER: S-EPMC12800430 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature