Circ_0001741 regulates proliferation and invasion in ESCC via the miR-194-5p/E2F3 axis.

ABSTRACT:

Purpose

Esophageal squamous cell carcinoma (ESCC) is a highly aggressive gastrointestinal malignancy. This study aims to investigate the role and molecular mechanism of a novel circular RNA, circ_0001741, in ESCC progression.

Methods

The circular structure of circ_0001741 was confirmed by RNase R and actinomycin D assays alongside divergent primer-based amplification targeting its backsplice junction (BSJ). Its expression was profiled using an ESCC tissue microarray. Functional effects of circ_0001741 on proliferation and invasion were assessed using CCK-8 and Transwell assays. The interactions within the circ_0001741/miR-194-5p/E2F3 axis were validated through dual-luciferase reporter, RNA pull-down, and rescue experiments.

Results

Circ_0001741 was significantly upre

SUBMITTER: Wang Y 

PROVIDER: S-EPMC12801454 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature