Rewiring central metabolism in Komagataella phaffii for efficient mannose synthesis.

ABSTRACT:

Background

Mannose has wide-ranging applications but microbial fermentation remains underdeveloped compared to biotransformation for its production. The yeast Komagataella phaffii stands out as a premier synthetic biology platform, renowned for its safety profile and exceptional suitability for high-density fermentation. This established chassis organism is ideally positioned for large-scale mannose production through targeted rewiring of its mannose biosynthetic pathway via metabolic engineering.

Results

K. phaffii was metabolically engineered for efficient mannose production using a dual carbon source system: glycerol for biomass generation and glucose for mannose synthesis. To redirect carbon flux toward fructose-6-phosphate (F6P) accumulation at the glycolytic node, glyc

SUBMITTER: Zhao S 

PROVIDER: S-EPMC12801476 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature