Mechanisms of azole resistance in a clinical isolate of Candida tropicalis.

ABSTRACT: Azole resistance has been insufficiently investigated in the yeast Candida tropicalis. Here we determined the molecular mechanisms responsible for azole resistance in a clinical isolate of this pathogenic yeast. Antifungal susceptibility testing performed by a disk diffusion method showed resistance or markedly decreased susceptibility to azoles, which was confirmed by determination of MICs. Considering the relationship between azole susceptibility and the respiration reported for other yeast species, the respiratory activity of this isolate was investigated. Flow cytometry using rhodamine 123 and oxygraphy demonstrated an increased respiratory activity, which was not linked to an overexpression or increased number of copies of the mitochondrial genome. Among previously described resistanc

SUBMITTER: Vandeputte P 

PROVIDER: S-EPMC1280149 | biostudies-literature | 2005 Nov

REPOSITORIES: biostudies-literature