Molecular cross-talk via extracellular vesicles for the characterization of young subjects with type 1 diabetes unravels new potential markers of insulin resistance and double diabetes.

ABSTRACT:

Background

Insulin resistance (IR) is commonly calculated using a simple mathematical formula, the eGDR (estimated Glucose Disposal Rate), but in the paediatric type I diabetes (T1DM) population this value has provided contrasting information. We aimed to provide a clearer metabolic "fingerprint" in children with "double diabetes", focusing on the molecular cross-talk mediated by extracellular vesicles (EVs).

Methods

Paediatric patients were classified based on the eGDR value in: insulin-resistant (T1DM+, eGDR < 8 mg/Kg/min, n = 29) and non-insulin-resistant (T1DM-, eGDR > 8 mg/Kg/min, n = 35). Venous blood collected from them, and 30 healthy controls was used to obtain dried blood spots (DBS) for AAs and ACs analysis by FIA-MS/MS and for EV by a patented flow cytometry meth

SUBMITTER: Cufaro MC 

PROVIDER: S-EPMC12801652 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature