A Multienzyme Magnetic Nanocatalyst for Efficient, Sustainable Hydrolysis of Wastewater-Grown Microalgae Consortia.

ABSTRACT: The rigid cell walls of microalgal consortia (Scenedesmus and Tribonema) hinder the efficient release of intracellular bioproducts and present a significant challenge for sustainable biomass utilization. To address this challenge, a novel multienzyme magnetic nanocatalyst (ME-MNC) was synthesized to enhance enzymatic hydrolysis. It comprised cellulase, α-amylase, amyloglucosidase, and alcalase immobilized on amino-functionalized iron oxide nanoparticles. Optimization using Central Composite Design identified optimal parameters: 103.82 mM glutaraldehyde, 178.79 min cross-linking time, and 7.08 h immobilization time. The catalytic system demonstrated enzyme activity recoveries of 64.66% (cellulase), 67.02% (α-amylase), 43.40% (amyloglucosidase), and 81.14% (alcalase). Structura

SUBMITTER: Gupta S 

PROVIDER: S-EPMC12801979 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature