Evaluation of the Simple One-Step (SOS) stool method for Truenat MTB plus and Xpert MTB/XDR assay.

ABSTRACT: Stool is a recommended sample for the diagnosis of Mycobacterium tuberculosis (MTB) using Xpert MTB/RIF (Ultra) (GXU) in children, and the Simple One-Step (SOS) stool method is one of the recommended processing methods. We investigated modifications to the protocol of the SOS stool method to make it fit for testing stool with Truenat MTB Plus and MTB-RIF Dx (Truenat) and Xpert MTB/XDR (GXX). Experiments were conducted using stool spiked with different MTB concentrations and using modified versions of the SOS stool processing method. The established protocol was then validated on routine clinical stool samples from presumptive TB patients, comparing the performance of Truenat and GXX against GXU. The concordance for MTB detection between GXU and Truenat using 50, 100, and 150 mg of s

SUBMITTER: Haas Pd 

PROVIDER: S-EPMC12802141 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature