Synergistic regulatory mechanisms in glycolysis revealed by pathway transplantation.

ABSTRACT: Protein allostery, present in all three domains of life, is key to the regulation of metabolism by allowing fast and precise control of catalysis in response to cellular demands. While metabolic pathways are frequently equipped with multiple allosterically regulated catalytic steps, experimental studies often focus on a single step, failing to capture how regulations exerted at multiple steps interact with each other for tuning pathways. Using the nearly ubiquitous Embden-Meyerhof-Parnas pathway of glycolysis as a paradigm, the present study unveils a remarkable regulatory synergy between multiple allosteric proteins of a metabolic pathway and demonstrates its impact on cell survival in dynamic environments. By using complete pathway complementation, as well as single-gene complementation,

SUBMITTER: Knibbe E 

PROVIDER: S-EPMC12802204 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature