Modelling the metastatic immune microenvironment in triple-negative breast cancer.

ABSTRACT:

Background

This study uses the well-established 4T1.2 and 67NR mammary cancer cell lines as syngeneic immunocompetent implantable murine models of triple negative breast cancer.

Methods

Using pair-wise analysis, we compare the implantation route of cancer cells by conventional orthotopic injections in the mammary fat pad vs intraductal injection in the mammary ductal tree. We reveal that implantation methodology influences the inherent metastatic potential, tumour progression and the co-evolution of cancer cells and the tumour microenvironment.

Results

Intraductal injection accelerates tumour growth, especially in the non-metastatic 67NR model, and better mimics early tumourigenic events that preserve the tissue microenvironment crucial for tumour-stromal interactions

SUBMITTER: de la Fuente LR 

PROVIDER: S-EPMC12802248 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature