Differential contributions of β-tubulin isotypes to acentrosomal oocyte meiosis in C. elegans.

ABSTRACT: Oocyte meiotic spindles must achieve bipolarity and segregate chromosomes in the absence of centrosomes. Here we use high-resolution immunofluorescence microscopy and live imaging to investigate the differential contributions of β-tubulin isotypes (TBB-1 and TBB-2) to assembly and function of acentrosomal spindles in Caenorhabditis elegans oocytes. By combining strains with altered β-tubulin isotype composition with mutations affecting microtubule-crosslinking motor KLP-18 and/or mutations affecting katanin-mediated microtubule severing, we show that TBB-1 and TBB-2 make distinct contributions to promoting spindle bipolarity. Further, by measuring multiple spindle features in wild-type and β-tubulin isotype substitution strains, we reveal contributions of isotype composition to spin

SUBMITTER: Nsamba ET 

PROVIDER: S-EPMC12802399 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature