Integrated Single Cell Spatial Analysis Reveals Dysregulated Basal Progenitor Cells in Ulcerative Colitis Pathogenesis: A Multi Omics Study.

ABSTRACT:

Background and aims

Ulcerative colitis (UC), a chronic inflammatory bowel disease, causes persistent mucosal inflammation and epithelial dysfunction. Basal progenitor cells (BPCs), critical for intestinal regeneration and mucosal repair, exhibit altered behavior in UC. Despite extensive research into immune dysregulation, the spatial dynamics and functional roles of BPCs in UC remain poorly understood.

Methods

We utilized multi-omics integration, single-cell RNA sequencing (scRNA-seq), spatial transcriptomics (ST), and machine learning (ML) to analyze BPC dynamics in UC. scRNA-seq data from UC and healthy samples were processed to identify the cellular composition, functional gene matrixes, and differentiation trajectories. ST provided spatial expression patterns, and ML mod

SUBMITTER: Chen X 

PROVIDER: S-EPMC12802566 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature