Chromatin immunoprecipitation-based screen to identify functional genomic binding sites for sequence-specific transactivators.

ABSTRACT: In various human diseases, altered gene expression patterns are often the result of deregulated gene-specific transcription factor activity. To further understand disease on a molecular basis, the comprehensive analysis of transcription factor signaling networks is required. We developed an experimental approach, combining chromatin immunoprecipitation (ChIP) with a yeast-based assay, to screen the genome for transcription factor binding sites that link to transcriptionally regulated target genes. We used the tumor suppressor p53 to demonstrate the effectiveness of the method. Using primary and immortalized, nontransformed cultures of human mammary epithelial cells, we isolated over 100 genomic DNA fragments that contain novel p53 binding sites. This approach led to the identification and

SUBMITTER: Hearnes JM 

PROVIDER: S-EPMC1280257 | biostudies-literature | 2005 Nov

REPOSITORIES: biostudies-literature