A Transcriptional Atlas of Endothelial Cell Zonation Along the Pulmonary Vascular Tree.

ABSTRACT:

Background

The lung vasculature is comprised of a series of branching vessels extending from the main pulmonary artery to the alveolar capillaries, then back to the pulmonary veins. Lung endothelial cells (EC) exist along this continuum, exposed to gradients of shear stress, oxygen tension and pressure. Single cell RNA sequencing (scRNA-seq) has identified lung EC subsets, but many aspects of the vascular continuum, including vessel size and capillary polarity remain undefined from transcriptomic data.

Methods

We created an endothelial-enriched scRNA-seq dataset from the P3 mouse lung. Using diffusion pseudotime across all lung EC, we developed an analytical framework to delineate transcriptomic gradients and assign vessel-size scores to categorize individual endothelial cel

SUBMITTER: Sveiven SN 

PROVIDER: S-EPMC12803053 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature