DAP-seq Reveals Cluster-Situated Regulator Control of Numerous <i>Streptomyces</i> Natural Product Biosynthetic Genes.

ABSTRACT: Natural products (NPs) are a rich source of therapeutic and agricultural compounds. Unfortunately, many promising metabolites are not expressed under standard laboratory conditions. Deepening our understanding of the regulatory networks governing NP biosynthetic genes is essential for unlocking this hidden chemical diversity. Cluster-situated regulators (CSRs) are transcription factors involved in the regulation of NPs, but their full regulatory range has remained elusive due to limited genome-wide data. Using DNA Affinity Purification Sequencing (DAP-seq), we defined the predicted regulons for 84 CSR homologs across 78 Streptomyces strains. CSRs in this cohort exerted influence across multiple cellular processes, with particularly strong impacts on other transcription factors throu

SUBMITTER: Wilbanks LE 

PROVIDER: S-EPMC12803058 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature