Comparative gene annotation and orthology assignments across 301 species of Drosophilidae.

ABSTRACT: High-quality genome annotations are essential if we are to address central questions in comparative genomics, such as the origin of new genes, the drivers of genome size variation, and the evolutionary forces shaping gene content and structure. Here, we present protein-coding gene annotations for 301 species of the family Drosophilidae, generated using the Comparative Annotation Toolkit (CAT) and BRAKER3, and incorporating available RNA-seq and protein evidence. We take a comparative phylogenetic approach to annotation, with the aim of improving consistency and accuracy, and to generate a robust set of gene annotations and orthology assignments. We analyze our annotations using a phylogenetic mixed-model approach and find that gene number and CDS length exhibit moderate phylogenetic herita

SUBMITTER: Dhakad P 

PROVIDER: S-EPMC12803070 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature