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CadC activates pH-dependent expression of the Vibrio vulnificus cadBA operon at a distance through direct binding to an upstream region.

ABSTRACT: The Vibrio vulnificus cadBA genes were transcribed as a transcriptional operon by a single promoter, P(cadBA), which was activated by CadC in a pH-dependent manner. A direct interaction between CadC and the P(cadBA) DNA was demonstrated, and a CadC binding site centered at -233.5 was mapped by deletion analyses of P(cadBA) and confirmed by a DNase I protection assay.

SUBMITTER: Rhee JE

PROVIDER: S-EPMC1280309 | biostudies-literature | 2005 Nov

REPOSITORIES: biostudies-literature

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CadC activates pH-dependent expression of the Vibrio vulnificus cadBA operon at a distance through direct binding to an upstream region.

Rhee Jee Eun JE Kim Kun-Soo KS Choi Sang Ho SH

Journal of bacteriology 20051101 22

The Vibrio vulnificus cadBA genes were transcribed as a transcriptional operon by a single promoter, P(cadBA), which was activated by CadC in a pH-dependent manner. A direct interaction between CadC and the P(cadBA) DNA was demonstrated, and a CadC binding site centered at -233.5 was mapped by deletion analyses of P(cadBA) and confirmed by a DNase I protection assay. ...[more]

PMID: 16267313

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