Evolutionary conservation and innovations of RNA polymerase II transcription elongation factors.

ABSTRACT: In eukaryotes, transcription elongation factors (TEFs) associate with RNA Polymerase II (RNAPII) to facilitate gene expression and couple transcription to co-transcriptional processes, including chromatin regulation and RNA processing. To further our understanding of TEF biology, we developed a domain-centric analysis pipeline to perform a broad survey of ten TEF orthologs -- Paf1, Ctr9, Cdc73, Rtf1, Leo1, Spt4, Spt5, Spt6, Spn1, and Elf1 -- across the Tree of Life and analyze their evolutionary patterns in a structural context. We report evidence for all ten TEFs being present in the last eukaryotic common ancestor, indicating that mechanisms of TEF-mediated transcription regulation are both ancient and conserved. However, some early-diverging eukaryotic clades exhibit signs of altered TE

SUBMITTER: Francette AM 

PROVIDER: S-EPMC12803144 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature