Mechanistic insights into <i>E. coli</i> recovery from growth arrest.

ABSTRACT: Bacteria survive hostile conditions in clinically relevant conditions by shutting down protein synthesis, but how they restart growth remains poorly understood. Here, we use an E. coli ΔrimM strain, which exhibits a prolonged growth arrest, as a model to investigate how bacteria recover from this arrested state and restore protein synthesis. RimM is a conserved ribosome maturation factor for the 3'-major (head) domain of the 16S rRNA within the bacterial 30S subunit. The loss of RimM causes a significantly longer delay in recovery than other 30S maturation factors, including RbfA - the presumed primary factor in 30S maturation. Cryo-EM analysis of ΔrimM ribosomes revealed a delayed recruitment of ribosomal proteins to the 30S head domain and increased occupancy of the

SUBMITTER: Hassan A 

PROVIDER: S-EPMC12803275 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature