Optimizing Post-Transplantation Detection of Subcutaneously Transplanted Islets Using Dithizone Staining.

ABSTRACT:

Introduction

Pancreatic islet transplantation is a promising therapeutic strategy to restore insulin independence in patients with type 1 diabetes mellitus. In addition to primary human islets, human pluripotent stem cell-derived islets have shown clinical promise. However, optimizing transplantation sites and improving methods for standardized islet dosing and post-transplant graft localization are key limitations. The subcutaneous space offers an alternative site for transplantation due to its accessibility and minimally invasive nature; nevertheless, poor vascularization and difficulty locating engrafted islets limit its experimental utility. The use of dithizone, a zinc-chelating dye that selectively binds the high intracellular zinc concentrations of insulin-producing β-cells,

SUBMITTER: Lu J 

PROVIDER: S-EPMC12803282 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature