Molecular, Transcriptomic, and Proteomic Characterization of <i>Plasmodium</i> Infections that Evade Detection by Rapid Diagnostic Tests in Mizan Aman, Ethiopia.

ABSTRACT:

Background

Failure of rapid diagnostic tests (RDTs) to detect Plasmodium parasites in peripheral blood of individuals is a major barrier to successful case management and control of malaria in Ethiopia. Characterizing factors contributing to RDT failure is essential if malaria control and elimination strategies are to succeed.

Methods

We consented and enrolled 148 individuals with suspected malaria presenting to health clinics in Mizan Aman, Ethiopia. We administered a clinical questionnaire, diagnosed the presence of malaria parasites via RDT, and collected venous blood. Samples were assayed using molecular methods to detect parasite DNA, Plasmodium species, parasite load, and pfhrp2 and pfhrp3 gene deletions. RNA-seq libraries and LC-MS proteomi

SUBMITTER: Nikulkova M 

PROVIDER: S-EPMC12803298 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature