Characterising the Periodontal Granulation Tissue Using scRNAseq.

ABSTRACT:

Aim

To investigate the cellular composition and molecular mechanisms of periodontal granulation tissue formation using single-cell RNA sequencing (scRNA-seq), aiming to enhance the understanding of periodontal disease pathogenesis and identify potential targets for regenerative therapies.

Materials and methods

Granulation tissue samples were collected from patients undergoing periodontal surgery (n = 3). Fresh tissues were processed into single-cell suspensions and subjected to scRNA-seq. The data were integrated and compared with existing datasets from healthy gingiva and periodontal ligament. Computational analyses were performed and validated through immunofluorescence staining.

Results

Ten distinct cell clusters were identified across the samples. Granulation tiss

SUBMITTER: Zhu W 

PROVIDER: S-EPMC12803696 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature