A novel surface plasmon resonance approach to assess anti-dsDNA antibody kinetics and disease severity in systemic lupus erythematosus.

ABSTRACT:

Objectives

Anti-dsDNA antibodies are established biomarkers in systemic lupus erythematosus (SLE), yet the clinical utility of conventional assays remains limited by variable analytical performance. We evaluated a novel Fibre-Optic Surface Plasmon Resonance (FO-SPR) biosensor for real-time profiling of anti-dsDNA antibody-antigen interactions and investigated whether kinetic parameters are associated with disease severity, particularly lupus nephritis (LN).

Methods

Sera from 26 SLE patients (including 12 with LN), 14 disease controls and 16 healthy donors were analysed using FO-SPR biosensors coated with biotinylated dsDNA. The sensors generated real-time kinetic profiles from which maximum binding response (B _max), association rate and dissociation constan

SUBMITTER: Nagant C 

PROVIDER: S-EPMC12803748 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature