Proteomic analysis of HDL isolates reveals method-driven variability: An interlaboratory approach.

ABSTRACT: The high-density lipoprotein (HDL) is the most heterogeneous and protein-rich lipoprotein, and its complex proteome has been correlated with many distinct properties. However, the lack of a standardized approach for HDL isolation, combined with the fact that different methods capture overlapping, but distinct HDL subspecies make the establishment of a composition-function relationship a challenge. Key factors influencing HDL proteomic profile are the isolation methodology and the technical variability associated with it. Importantly, interlaboratory technical variability associated with HDL isolation methodologies and how it affects the HDL proteome has never been determined. Here, we used two common methods to isolate HDL particles, ultracentrifugation (UC) and immunoaffinity chromatograp

SUBMITTER: Gomes FA 

PROVIDER: S-EPMC12803808 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature