Single-cell analysis of heterogeneity in reverted hiPSC-derived human hepatic stellate cells.

ABSTRACT:

Background & aims

Activated HSCs are known to drive fibrogenesis, but their fate following injury resolution remains unclear. We aimed to investigate whether human activated HSCs revert to a less activated state, and to characterize features of such reversion using a human induced pluripotent stem cell (hiPSC)-derived multicellular liver model.

Methods

We used a hiPSC-derived liver culture containing hepatocytes, HSCs, and macrophages. HSCs were activated by HCV infection or a lipotoxic milieu modeling metabolic dysfunction-associated steatotic liver disease (MASLD) and subjected to injury resolution through antiviral treatment or replacement with a healthy medium. Reverted HSCs were characterized via gene expression profiling, functional assays, and single-cell RNA sequenci

SUBMITTER: Wang X 

PROVIDER: S-EPMC12803900 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature