A Pilot Study Profiling Aqueous Humor-Derived Exosomal MicroRNA in Primary Open-Angle and Exfoliation Glaucoma.

ABSTRACT:

Objective

To profile aqueous humor (AH)-derived exosomal microRNAs (miRNAs) in primary open-angle glaucoma (POAG) and exfoliation glaucoma (XFG) and investigate their potential as diagnostic biomarkers and modulators of extracellular matrix (ECM) remodeling in glaucoma pathogenesis.

Design

Prospective, comparative, observational pilot study.

Subjects and controls

Aqueous humor was obtained from 21 patients with POAG, 24 patients with XFG, and 14 healthy donors.

Methods

Exosomes were isolated from pooled AH samples of patients with POAG, XFG, and healthy donors for small RNA sequencing. miR-29a, an ECM-targeting miRNA, was selected for functional validation in transforming growth factor-beta 2 (TGF-β2)- or dexamethasone (Dex)-induced collagen deposition models u

SUBMITTER: Zou Y 

PROVIDER: S-EPMC12803933 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature