Design of a microsphere-based high-throughput gene expression assay to determine estrogenic potential.

ABSTRACT: Recently gene expression studies have been multiplied at an accelerated rate by the use of high-density microarrays. By assaying thousands of transcripts at a time, microarrays have led to the discovery of dozens of genes involved in particular biochemical processes, for example, the response of a tissue/organ to a given chemical with therapeutic or toxic properties. The next step in these studies is to focus on the response of a subset of relevant genes to verify or refine potential therapeutic or toxic properties. We have developed a sensitive, high-throughput gene expression assay for this purpose. In this assay, based on the Luminex xMAP system, carefully selected oligonucleotides were covalently linked to fluorescently coded microspheres that are hybridized to biotinylated cRNA follow

SUBMITTER: Naciff JM 

PROVIDER: S-EPMC1280396 | biostudies-literature | 2005 Sep

REPOSITORIES: biostudies-literature