Protocol for targeted gene manipulation and thermogenic evaluation in mouse brown adipocytes.

ABSTRACT: Adeno-associated viruses (AAVs) are versatile, non-integrating vectors for in vivo gene delivery. We present a reproducible workflow for generating Cre-dependent FLEX-AAVs, quantifying viral titer, and performing localized injections for cell-type-specific transgene expression in mice. The protocol also details the assessment of thermogenic capacity in genetically modified brown adipocytes using Clark-type electrode respirometry. For complete details on the use and execution of this protocol, please refer to Bunk et al.¹.

SUBMITTER: Bunk J 

PROVIDER: S-EPMC12803982 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature