Regulating protein glycosylation modification enhances the synthesis of taxadiene in <i>Saccharomyces cerevisiae</i>.

ABSTRACT: Utilizing synthetic biology techniques to construct recombinant engineered cells for the synthesis of paclitaxel and its key precursors has emerged as an effective method to address the supply-demand imbalance and protect rare plant resources. Taxadiene, a critical precursor of paclitaxel, exhibits limited yield in eukaryotic systems, constraining its biosynthetic potential. Previous research has demonstrated that glycosylation modifications in Saccharomyces cerevisiae substantially impact the regulation of exogenous protein expression. In this study, we found that knocking out endogenous protein glycosylation genes in the chassis significantly improved the expression of heterologous proteins, especially the key taxadiene synthase (TS), and thereby increased the yield of taxadiene.

SUBMITTER: Zhang C 

PROVIDER: S-EPMC12804127 | biostudies-literature | 2026 Jun

REPOSITORIES: biostudies-literature