Dynamic control of bacterial antiphage defense through the CdnG-Cap5 cyclic oligonucleotide-based antiphage pathway in Vibrio cholerae.

ABSTRACT: The cyclic oligonucleotide-based antiphage signaling system (CBASS) is a key defense mechanism that protects bacteria against viral infections, exhibiting functional homology with the eukaryotic cyclic GMP-AMP synthase-stimulator of interferon gene innate immune pathway. The CBASS immune pathway in Vibrio cholerae, a significant human pathogen, positions it as a promising target for novel therapeutic strategies. Here, we report the biochemical and structural characterization of the CdnG-Cap5 CBASS system from V. cholerae, a highly abundant and representative clade G system. Our results elucidate the mechanistic basis of CBASS immunity, from second messenger synthesis to effector activation. We demonstrate that VcCdnG produces 3'2'-cyclic GMP-AMP as a bacterial second messenger, which speci

SUBMITTER: Ye F 

PROVIDER: S-EPMC12804361 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature