Microwave-assisted synthesis of new triangulenium dyes for lifetime imaging microscopy (FLIM).

ABSTRACT: We introduce a novel microwave-assisted method for the rapid and efficient synthesis of triangulenium dyes, enabling the creation of a broader range of these fluorophores, which are characterized by very long fluorescence lifetimes. We prepared different azadioxatriangulenium (ADOTA) and diazaoxatriangulenium (DAOTA) derivatives and thoroughly characterized their photophysical properties. We investigated their solvatochromic properties and the effects of the solvent, aided by computational chemistry results. The new ADOTA and DAOTA dyes were applied in multiple in cellulo microscopy modalities, including confocal, fluorescence lifetime imaging microscopy (FLIM), and super-resolution imaging. ADOTA derivatives predominantly accumulated in mitochondria, while DAOTAs showed pronounced nuclear

SUBMITTER: Viedma-Barba C 

PROVIDER: S-EPMC12804676 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature