Microbiome-dependent functional responses to structurally distinct oligosaccharides revealed by metaproteomics.

ABSTRACT: Dietary oligosaccharides are prebiotics that fuel gut microbes, but individual microbiomes may respond differently depending on oligosaccharide structures as well as microbiome composition and function. The extent to which specific gut microbial communities exhibit personalized functional responses to distinct oligosaccharides remains underexplored. We applied a standardized ex vivo microbiome culture, called RapidAIM, coupled with metaproteomics to examine how six structurally diverse oligosaccharides affect the gut microbiota functional response. Our study shows that while human gut microbiomes share some commonalities in utilizing oligosaccharides (e.g. prioritizing dietary fibers over mucin), the fine-scale metabolic and taxonomic responses are highly individualized. Such findings unde

SUBMITTER: Zhang A 

PROVIDER: S-EPMC12804693 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature