Hybrid 3D-printed/electrospun scaffolds drive myogenic differentiation of mesenchymal stem cells (MSCs).

ABSTRACT: The development of functional scaffolds that support cell differentiation and tissue regeneration remains a major challenge in regenerative medicine. In this study, we designed and fabricated hybrid scaffolds (HS) composed of poly(lactic-co-caprolactone) (PLA-PCL) by integrating 3D extrusion-based printing and electrospinning techniques. Two distinct 3D-printed architectures were explored-aligned filaments and grid patterns-which were subsequently coated with a layer of electrospun nanofibers to better mimic the anisotropic and hierarchical structure of native skeletal muscle tissue. Bone marrow porcine mesenchymal stem cells (p-MSCs) were seeded onto the scaffolds and cultured under myogenic conditions. To evaluate the progression of myogenic differentiation, we assessed the expression of

SUBMITTER: Pisani S 

PROVIDER: S-EPMC12804726 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature