Effect of carbohydrate substrates on growth and enterotoxin gene expression in Bacillus cereus (pacificus).

ABSTRACT: Bacillus cereus sensu lato is a common contaminant of improperly stored food and human milk, capable of causing severe emetic and diarrheal diseases, particularly in infants and immunocompromised individuals. In this in vitro study, the effects of various temperatures and carbohydrate substrates on the growth of B. cereus (pacificus) strain ATCC 10987 and its expression of enterotoxin genes were explored. Bacterial growth at several temperatures and with six different carbohydrate substrates was evaluated. In cultures grown with various substrates at 37 °C, selected metabolites were analyzed using capillary electrophoresis and RNA-seq transcriptomic profiling was performed. At the same temperature, glucose, fructose, sucrose, and xylitol-enriched environments were preferable for the bacter

SUBMITTER: Vyklicka K 

PROVIDER: S-EPMC12804731 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature