Construction, evaluation, and applications of renal barrier-on-a-chip system.

ABSTRACT: Organ-on-a-chip (OoC) technology offers a transformative approach to modeling the human renal barrier, overcoming limitations of traditional animal and two-dimensional cell models. This review systematically outlines the construction and evaluation of renal barrier biochips, focusing on the glomerular filtration barrier (GFB), tubular reabsorption barrier (TRB), and collecting duct regulatory barrier (CDRB). OoC platforms integrate biomimetic materials, simulate dynamic microenvironments, and use multicellular co-culture strategies. This enables them to closely replicate the structural and functional characteristics of renal barriers. Key evaluation metrics-including structural biomimicry, barrier integrity, and active transport functions-are discussed to validate model performance. The te

SUBMITTER: Naren T 

PROVIDER: S-EPMC12805313 | biostudies-literature | 2026 May

REPOSITORIES: biostudies-literature