Cooperative ligand binding in a bacterial heme-based oxygen sensor.

ABSTRACT: Bacteria modulate essential phenotypes in response to external signals such as the availability of molecular oxygen (O₂). A class of direct O₂-sensing heme proteins, globin-coupled sensors, have been implicated in O₂-dependent regulation of pathogenic phenotypes, including biofilm formation, motility, and virulence. While cooperative O₂ binding is well known in both mammalian and prokaryotic hemoglobins, cooperative ligand binding previously has not been observed in bacterial sensor globins. This study explores the O₂-dependent allosteric communication between globin domains in the globin-coupled sensor protein from Pectobacterium carotovorum (PccGCS) through equilibrium O₂-binding measurements, X-ray crystallography, resona

SUBMITTER: Hoque NJ 

PROVIDER: S-EPMC12805357 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature